Identification of additional fire blight resistance candidate genes following MinION Oxford Nanopore sequencing and assembly of BAC clone spanning the Malus fusca resistance locus

Abstract Pyramiding different fire blight resistance genes and QTLs in future apple cultivars is the most eco-friendly way to combat this disease. Identification of strong donors, introgression their into breeding material are a continuing effort programs. Thus, enormous been put research understand host – pathogen interactions mechanisms found Malus . The crabapple fusca (accession MAL0045) highly resistant blight, although strain-dependent, MAL0045 not overcome by any known strain Erwinia amylovora date. A locus ( FB_Mfu10 ) was fine mapped an interval 0.33 Centimorgan (cM) on linkage group (LG) 10 using 1888 progenies. Subsequently, single bacterial artificial chromosome (BAC) clone (46H22), which harbours -resistance alleles, identified BAC library sequenced MiSeq illumina leading assembly 45 contigs. Analyses sequence 46H22 led identification receptor-like kinase candidate gene. Here, we report about resequencing MinION Oxford Nanopore successfully assembled sequences contig, allowed for identifying additional genes.